Research Associate, Antibody Discovery and Protein Engineering

NextCure is a clinical-stage biopharmaceutical company committed to discovering and developing novel, first-in-class immunomedicines to treat cancer and other immune-related diseases by restoring normal immune function. We are seeking a motivated individual with hands-on experience in mouse immunizations, tissue culture and molecular cloning for antibody discovery towards our novel targets.


  • Immunize mice, process rodent tissue samples and generate hybridoma fusions from B cells and myeloma cells
  • Characterize antibody/ligand binding by ELISA, FACS, HTRF, SPR/BLI assays
  • Recover and analyze gene sequences from hybridoma clones (PCR, molecular cloning, gene and protein sequence analysis, etc)
  • Analyze antibody stability and liabilities, express optimized constructs
  • Generate, interpret, analyze data and present to project teams
  • Independently execute experiments in a collaborative environment


  • Bachelor’s Degree in Biochemistry/Molecular Biology/Immunology with 3-5 years of relevant experience or Master’s Degree with 1-3 years of relevant experience.
  • Background in antibody function and immunology.
  • Hands-on experience in sterile tissue culture techniques working with cell lines.
  • Practical experience with molecular biology (ie. PCR, gel electrophoresis, transformations)
  • Experience characterizing antibodies and proteins using automation and high-throughput screens.
  • Enthusiasm to learn new skills and discover new technologies.
  • Exceptional record keeping, analytical and troubleshooting skills.
  • Highly organized and able to operate in a fast-paced setting.
  • Excellent communication skills and ability to work in a multidisciplinary, team-oriented environment

NextCure is an Equal Opportunity Employer and offers a competitive salary and benefits package in a scientifically engaged team work environment.

Job Types: Full-time, Contract


  • hybridoma: 1 year (Preferred)
  • relevant: 1 year (Preferred)

Contract Renewal:

  • Likely

Work Location:

  • One location


  • Health insurance
  • Dental insurance
  • Vision insurance


  • Monday to Friday